Part:BBa_K5299200:Design

P3.1 - BBa_B0030 - BBa_I746916 - BBa_B0015

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 259
  

Design Notes

Created using the Golden Braid method, that utilises type IIS enzymes. We used BsmBI to create Level 0 constructs, and BsaI for Level α constructs.

Source

Promoter is BBa_K4583008, originally from [1].
RBS is BBa_B0030.
sfGFP is BBa_I746916.
Terminator is BBa_B0015.

References

• [1]: Jaishankar, J., & Srivastava, P. (2020). Strong synthetic stationary phase promoter-based gene expression system for Escherichia coli. Plasmid, 109 (102491), 102491. doi:10.1016/j.plasmid.2020.102491